Based on primery screening results the most perspective molecules could be tested on CPE assay to measuse general antiviral activity. Also this molecules could be screened on enzymatic assays for IC50 measure.
Cell line: Vero E6
Virus (origin/strain): SARS-Cov-2
Treatment: 4 days
Infection: 100 and/or 1000 TCID50
Detection: MTT and/or microscope
Real-time PCR for SARS-Cov-2 RNA: yes, Ct
Format: 96-well, single compound test
Screening the most perspectives compounds for measuring its IC50 on one of enzymatic assays: 3CL, PLpro, RdRp, ACE2-S1.