Primary Screen

The CoV in-vitro screening platform has collected in itself the all known and available assays on main SARS-CoV-2 targets. Moreover, we can develop an individual assay for your needs. Assays are also available in HTS format for up to 5000 compounds per day.

3CL (Mpro)

3Clpro Origin: SARS-Cov-2, recombinant, expressed in E.Coli

Substrate: internally quenched fluorogenic (FRET) substrate for SARS-CoV-2 3CLpro protease

Detection: FRET, exc/em=360 nm/460 nm

Format: 384-well, single compound test or HTS format up to 5000 cmpds/day

PLpro

PLpro Origin: SARS-Cov-2, recombinant, expressed in E.Coli

Substrate: internally quenched fluorogenic (FRET) substrate for SARS-CoV-2 PLpro protease

Detection: FRET, exc/em=360 nm/460 nm

Format: 384-well, single compound test or HTS format up to 5000 cmpds/day

RdRp

RdRp Origin: SARS-CoV-2, recombinant, expressed in HEK293

Thare are two types of assays: Enzymatic and Binding. The type of assay depends on the research goals

ACE2-S1

The assay is useful for screening of ACE2 binding to SARS-CoV-2 Spike S1.

Type: Binding

Format: 384-well, single compound test or HTS format up to 5000 cmpds/day

Detection: TR-FRET, exc/em=340 nm/620 nm

The equipment we use
Tecan M1000 Pro
Biomek 384FX
Biomek 2000